Evaluation of the immunomodulatory activity of copalic acid from Copaiba resin

Authors

  • Bruno Damasceno Bahia Federal University of Juiz de Fora
  • Kézia Cristine Barbosa Ferreira Federal University of Juiz de Fora
  • Danilo de Souza Costa Federal University of Juiz de Fora
  • Chislene Pereira Vanelli Hospital Universitário da UFJF
  • Frederico Pittella Silva Federal University of Juiz de Fora
  • Ademar Alves da Silva Filho Federal University of Juiz de Fora
  • Ana Cláudia Chagas de Paula Ladvocat Federal University of Juiz de Fora
  • Jose Otavio do Amaral Correa UFJF https://orcid.org/0000-0003-2231-1160

Abstract

Introduction: The immune system has the function of maintaining the body's homeostasis, acting in balance against pathogens and tumors. Inappropriate immune responses can promote the onset of diseases, including autoimmune diseases. The immune response can be modulated through traditional drugs, such as anti-inflammatories and immunosuppressants, which are sometimes associated with high costs and the occurrence of serious adverse effects. In this scenario, natural substances can be an alternative for the development of new immunomodulatory drugs. Copalic acid (CA), present in Copaiba sp resin, is a natural diterpene with a potential immunomodulatory activity. Objective: To evaluate the in vitro immunomodulatory effects of CA. Material and Methods: After isolated and identified from copaiba resin, the cytotoxicity of AC was evaluated by MTT and Trypan blue methods. Cell proliferation, nitric oxide (NO) production (by Griess method), and cytokine production (by ELISA) were evaluated in cells from C57BL/6 mice. Results: CA did not show a reduction in cell viability at concentrations of 75, 50, 25, 10 and 5 μM. CA (75 and 50 µM) inhibited cell proliferation stimulated by concanavalin A and, at 75 µM, reduced NO production. CA was also able to reduce the production of cytokines IL-1β, IL-6 and IL-12, mainly at 75 µM. Conclusion: CA showed an in vitro immunomodulatory effect, reinforcing its anti-inflammatory potential. However, further studies must be carried out to clarify its mechanism of action.

 

Keywords: Immunomodulation; natural compounds; New therapeutics drugs.

Author Biography

Jose Otavio do Amaral Correa, UFJF

Possuo graduação em Farmácia e Bioquímica pela Universidade Federal de Juiz de Fora (UFJF, 1998), Mestrado em Ciências pela Fundação Oswaldo Cruz (FIOCRUZ/RJ, 2003), Doutor em patologia pela Universidade Federal Fluminense (UFF/Niterói, 2008). Sou Gerente de Ensino e Pesquisa do Hospital Universitário da UFJF, professor Associado II do Departamento de Ciências Farmacêuticas da Universidade Federal de Juiz de Fora. Neste Departamento leciono as disciplinas de Bioquímica aplicada às Análises Clínicas, Imunologia aplicada às Análises Clínicas e Orientador do estágio e tutor em residência em análises clínicas no Hospital Universitário da UFJF. Na pós-graduação strictu sensu Fui Coordenador e sou membro Permanente do Programa de Pós-Graduação em Ciências Farmacêuticas (Mestrado e doutorado em Ciências Farmacêuticas / UFJF / Capes 4). Membro permanente Programa de Pós-graduação em Saúde (mestrado e doutorado) no Núcleo de Pesquisa em Imunopatologia e Imunologia Clínica - (Faculdade de Medicina / UFJF / Capes 5). Possui experiência na área de Imunologia celular, imunofarmacologia e bioquímica clínica. Em Educação, avaliador do INEP/MEC, com experiência em avaliação de cursos de graduação e institucional, atuou como membro da Comissão Técnica de Acompanhamento de Avaliação do INEP/MEC representante da área da Farmácia na CTAA/MEC(2013-2015). Na gestão, fui Diretor da Faculdade de Farmácia da UFJF (2014-2018). Também fui Coordenador do curso de Farmácia da UFJF 2012 a 2014 e fui Coordenador do Programa de Pós-Graduação em Ciências Farmacêuticas da Faculdade de Farmácia / UFJF. É membro de corpo editorial de revista internacional especializada e revisor de periódicos nacionais e internacionais. Publiquei, desde 1999, 47 artigos em periódicos,sendo meu fator H = 14 (SCOPUS), H= 14 e índice i10 = 18 (https://www.adscientificindex.com/scientist/jose-otavio-do-amaral-correa/897285). Tenho diversas formas de citação em meus trabalhos (CORREA, J.O.A.; Corrêa, José Otávio; OTÁVIO DO AMARAL CORRÊA, JOSÉ; DO AMARAL CORRÊA JOSÉ, O; DO AMARAL CORRÊA, JOSÉ OTAVIO; José Otávio do Amaral Corrêa; Corrêa, José Otávio do Amaral; DO AMARAL CORRÊA, JO; AMARAL CORRÊA, JOSÉ OTÁVIO DO; CORRÊA, JOSÉ; CORRÊA, JOSÉ O.A.). Em bases de pesquisadores possuo o ResearcherID: AAE-8042-2021 ( http://www.researcherid.com/rid/AAE-8042-2021), Orcid: 0000-0003-2231-1160 (https://orcid.org/0000-0003-2231-1160), Scopus Author Identifier: 36626974500 e Google Scholar (https://scholar.google.com.br/citations?hl=pt-BR&user=l9eqYukAAAAJ). 

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Published

2023-12-31

How to Cite

Bahia, B. D., Ferreira, K. C. B., Costa, D. de S., Vanelli, C. P., Silva, F. P., da Silva Filho, A. A., Ladvocat, A. C. C. de P., & Correa, J. O. do A. (2023). Evaluation of the immunomodulatory activity of copalic acid from Copaiba resin. REVISTA CIENTÍFICA DA FAMINAS, 18(2), 1–13. Retrieved from https://periodicos.faminas.edu.br/index.php/RCFaminas/article/view/716

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Vol. 18 No. 2 (2023): REVISTA CIENTÍFICA DA FAMINAS

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